Separation of plant pigments by columna chromatography
Keywords:
Plant pigments, column chromatography, applicationsAbstract
This research is focused on the analysis of plant pigments extracted from spinach and chard using column chromatography, an efficient tool to separate and characterize compounds. Plant pigments, such as chlorophyll and carotenoids, are essential both for their biological function and for their diverse applications in industry. Extracts of spinach and chard were employed, using solvents such as petroleum ether and acetone to facilitate the extraction of these compounds. The results obtained showed that column chromatography allowed efficient separation of pigments, identifying mainly chlorophyll A and B, as well as carotenoids. It was observed that spinach, due to its high concentration of chlorophyll, produced a greater amount of this pigment, unlike chard, which in addition to chlorophyll, presented a greater diversity of pigments, including carotenoids, flavonoids and xanthophylls. This suggests that chard may have a broader potential for bioactive compounds. These findings highlight the ability of column chromatography to separate plant pigments, which opens the door to further research into their properties and applications in areas such as food, pharmacology and cosmetics.
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